Nanobiotecnologia

(Unitat: Proteòmica Aplicada i Enginyeria de Proteïnes)

Coordinador

Antoni Villaverde Corrales
Tel. 93 581 2148 - Tel. Laboratori  93 581 28 64
E-mail  Antoni.Villaverde@uab.es
 

Activitats de recerca

    * Disseny i producció bacteriana de proteïnes multifuncionals recombinants.

    * Desenvolupament de vectors no-vírics per a teràpia gènica.

    * Disseny de nous reactius enzimàtics per al diagnòstic de malalties infeccioses

    * Estudi de respostes de estrès cel·lular a la producció recombinant

    * Estudi de l’agregació proteica en bacteris

 
Altres activitats científiques

Edició de la revista Micro Cell Fact 

 

 

Cap de Grup:

Villaverde Corrales, Antonio

Membres:

 

2009

1 Vázquez, E. Ferrer-Miralles, N., Mangues, R, Corchero, JL, Schwartz Jr, S, Villaverde, A.  2009. Modular protein engineering in emerging cancer therapies. Current Pharmaceutical Design. 15: 893-916.

2 Olivier Laczka, Rosa María Ferraz, Neus Ferrer-Miralles, Antonio Villaverde, Francesc Xavier Muñoz and F. Javier del Campo. 2009. Fast electrochemical detection of anti-HIV antibodies: coupling allosteric enzymes and disk microelectrode arrays. Analytica Chimica Acta 641: 1-6.

3 Saccardo, P., Villaverde, A, Gonzalez-Montalbán, N. 2009. Peptide-mediated DNA condensation for non-viral gene therapy. Biotechnology Advances. 27:432-438.

4 Rosa María Ferraz, Escarlata Rodríguez-Carmona, Neus Ferrer-Miralles, Andreas Meyerhans, Antonio Villaverde. 2009. Screening HIV-1 antigenic peptides as receptors for antibodies and CD4 in allosteric nanosensors. Journal of Molecular Recognition. 22: 255-260.

5 Martínez-Alonso, M, Gonzalez-Montalbán, N, García-Fruitós, E, Villaverde, A. 2009. Learning about protein solubility from bacterial inclusion bodies. Microbial Cell Factories 8:4.

6 Ferrer-Miralles, N, Domingo-Espin, J., Corchero, JL, Vazquez, E., Villaverde, A. 2009. Microbial factories for recombinant pharmaceuticals. Microbial Cell Factories, 8:17

7 García-Fruitós, E, Rodríguez-Carmona, E, Diez-Gil, C, Ferraz, RM, Vázquez, E,, Corchero, JL, Cano-Sarabia, M, Ratera, I, Ventosa, N,Veciana, J, Villaverde, A. 2009. Surface Cell Growth Engineering Assisted by a Novel Bacterial Nanomaterial. Advanced Materials, 21: 4249-4253.

8 Sanchez-Aparicio, MT, Rosas, MF, Ferraz RM, Delgui, L, Veloso, JJ, Blanco E, Villaverde A, Sobrino F. 2009. Discriminating foot-and-mouth disease virus-infected and vaccinated animals through beta-galactosidase allosteric biosensor. Clin Vaccine Immunol. 16: 1228-1235.

9 Corchero, JL, Villaverde A. 2009. Biomedical applications of distally controlled magnetic nanoparticles. Trends in Biotechnology. 27:468-476

10 Martínez-Alonso M, Toledo-Rubio V, Noad R, Unzueta U, Ferrer-Miralles N, Roy P, Villaverde A. 2009. Re-hosting bacterial chaperones for high- quality protein production. Applied and Environmental Microbiology. 75: 7850–7854.

 

2010

1 Esther Vazquez, Mónica Roldán, César Diez-Gil, Ugutz Unzueta, Joan Domingo-Espín, Juan Cedano, Oscar Conchillo, Imma Ratera, Jaume Veciana, Xavier Daura, Neus Ferrer-Miralles Antonio Villaverde. 2010. Protein nanodisk assembling and intracellular trafficking powered by an arginine-rich (R9) peptide. Nanomedicine 5: 259-268.

2 Martínez-Alonso M, Villaverde A. Ferrer-Miralles N. 2010. Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production. Bioengineered Bugs. 1(2): 1-3.

3 García-Fruitós E, Villaverde A. 2010. Friendly production of bacterial inclusion bodies. Korean Journal of Chemical Engineering. 27: 385-389.

4 Verónica Toledo-Rubio, Esther Vazquez, Gemma Platas, Joan Domingo-Espín, Ugutz Unzueta, Elena García-Fruitós, Neus Ferrer-Miralles, Antonio Villaverde. 2010. Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay. Journal of Biomolecular Screening 15: 453-457.

5 Martínez-Alonso M, Gómez-Sebastián S, Escribano JM, Saiz JC, Ferrer-Miralles N, Villaverde A, 2010. DnaK/DnaJ-assisted recombinant protein production in Trichoplusia ni larvae. Applied Microbiology and Biotechnology 86: 633-639.

6 César Díez-Gil, Sven Krabbenborg, Elena García-Fruitós, Esther Vázquez, Escarlata Rodríguez-Carmona, Rosa M. Ferraz, Imma Ratera, Nora Ventosa, Joaquín Seras, Olivia Cano, Antonio Villaverde and Jaume Veciana. 2010. The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation. Biomaterials 31: 5805-5812.

7 García-Fruitós, E., Seras, J. Vazquez, E., Villaverde, A. 2010. Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering. Nanotechnology 21: 205101.

8 Escarlata Rodríguez-Carmona and Antonio Villaverde. 2010. Nanostructured bacterial materials in innnovative medicines. Trends in Microbiology. 18: 423-430.

9 Mónica Martínez-Alonso, Elena García-Fruitós, Neus Ferrer-Miralles, Ursula Rinas, Antonio Villaverde. 2010. Side effects of chaperone gene co-expression in recombinant protein production. Microbial Cell Factories, 9: 64.

10 Vazquez E, Cubarsí R, Unzueta U, Roldán M, Domingo-Espín J, Ferrer-Miralles, N, Villaverde A. 2010. Internalization and kinetics of nuclear migration of protein-only, argininie-rich nanoparticles. Biomaterials 31: 9333-9339. Figure 1 in Poster 2010 – The Year in Images. Biomaterials.

11 García-Fruitós E. Inclusion bodies: a new concept. 2010. Microbial Cell Factories. 9: 80-82

12 Villaverde, A. 2010. Nanotechnology, bionanotechnology and microbial cell factories (Editorial). Microbial Cell Factories.  9:53

13 Escarlata Rodríguez-Carmona, Olivia Cano-Garrido, Joaquin Seras-Franzoso, Antonio Villaverde, Elena García-Fruitós. Isolation of cell-free bacterial inclusion bodies. Microbial Cell Factories 9:71.

14  Vazquez E and Villaverde A. 2010. Engineering building blocks for self-assembling protein nanoparticles Microbial Cell Factories.  9:101

 

2011

1 Martin Dragosits, Gianni Frascotti, Lise Bernard-Granger, Felícitas Vázquez, Maria Giuliani, Kristin Baumann, Escarlata Rodríguez-Carmona, Jaana Tokkanen, Ermenegilda Parrilli, Marilyn G. Wiebe, Renate Kunert, Michael Maurer, Brigitte Gasser, Michael Sauer, Paola Branduardi, Tiina Pakula, Markku Saloheimo, Merja Penttilä, Pau Ferrer, Maria Luisa Tutino, Antonio Villaverde, Danilo Porro and Diethard Mattanovich. 2011. Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis. Biotechnology Progress 27: 38-46.

2 Elena García-Fruitós, Esther Vazquez, Nuria Gonzalez-Montalbán, Neus Ferrer-Miralles, Antonio Villaverde. 2011. Analytical approaches for assessing aggregation of protein biopharmaceuticals. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 10: 1530-1536.

3 Joan Domingo-Espín, Esther Vazquez, Javier Ganz, Oscar Conchillo, Elena García-Fruitós, Juan Cedano, Ugutz Unzueta, Valérie Petegnief, Nuria Gonzalez-Montalbán, Anna M Planas, Xavier Daura, Hugo Peluffo, Neus Ferrer-Miralles, Antonio Villaverde. 2011. The nanoparticulate architecture of protein-based artificial viruses is supported by protein-DNA interactions. Nanomedicine: 6; 1047-1061.

4 José Luis Corchero, Rosa Mendoza, Julia Lorenzo, Esther Vázquez, Neus Ferrer-Miralles, and Antonio Villaverde. Integrated approach to optimize transient gene expression in mammalian cells: production of a recombinant human alpha-galactosidase A. 2011 Biotechnology Progress. 27:1206-17.

5 Escarlata Rodríguez-Carmona, Antonio Villaverde, Elena García-Fruitós. 2011. How to break recombinant bacteria: does it matter? Bioengineered Bugs 2: 222-225.

6 García-Fruitós, E; Sabaté, R; Sánchez de Groot, N; Villaverde, A; Ventura, S. 2011. Biological role of bacterial inclusion bodies: a model for amyloid aggregation. FEBS Journal. 278:2419-2427.

7 Patnaik S, Tripathi SK, Goyal R., Arora A, Mitra K, Villaverde, A, Vazquez E., Shukla Y, Kumar P and Gupta KC. 2011. Polyethylenimine-polyetheneglycol-bis(aminoethylphosphate) nanoparticles mediated efficient DNA and siRNA transfection in mammalian cells. Soft Matter, 2011, 7: 6103-6112

 8 Ferrer-Miralles N, Corchero JL, Kumar P, Cedano JA, Gupta KC, Villaverde A and Vazquez E.  2011. Biological activities of histidine-rich peptides; merging Biotechnology and Nanomedicine. Microbial Cell Factories, 10:101.

9 Esther Vazquez, Jose Luis Corchero and Antonio Villaverde, 2011. Post-production protein stability: trouble beyond the cell factory. Microbial Cell Factories 2011, 10:60.

10 Gemma Platas, Escarlata Rodríguez-Carmona, Elena García-Fruitós, Olivia Cano-Garrido and Antonio Villaverde. 2011. Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control. Microbial Cell Factories, 10: 79.

11 Buckle AM, Bate MA, Androulakis S, Cinquanta M, Basquin J, Bonneau F, Chatterjee DK, Cittaro D, Gräslund S, Gruszka A, Page R, Suppmann S, Wheeler JX, Agostini D, Taussig M, Taylor CF, Bottomley SP, Villaverde A, de Marco A. 2011. Recombinant protein quality evaluation: proposal for a minimal information standard. Standards in Genomic Sciences 5: 195-197.

12 Corchero JL, Cedano J. Self-assembling, protein-based intracellular bacterial organelles: emerging vehicles for encapsulating, targeting and delivering therapeutical cargoes.

Microb Cell Fact. 2011 Nov 3;10:92. doi: 10.1186/1475-2859-10-92.

 

2012

 1 Benelmekki M, Xuriguera E, Caparros C, Rodríguez-Carmona E, Mendoza R, Corchero JL, Lanceros-Mendez S, Martinez LM. Design and characterization of Ni2+ and Co2+ decorated Porous Magnetic Silica spheres synthesized by hydrothermal-assisted modified-Stöber method for His-tagged proteins separation. J Colloid Interface Sci. 2012 Jan 1;365(1):156-62. doi: 10.1016/j.jcis.2011.09.051.

2  Garcia-Fruitos E. Lactic Acid Bacteria: a promising alternative for recombinant protein production. 2012. Microbial Cell Factories. 11:157

3 Hugo Peluffo, Daniela Alí-Ruiz, Aroa Ejarque-Ortíz, Victor Heras-Alvarez, Emma Comas-Casellas, Agueda Martínez-Barriocanal, Andres Kamaid, Damiana Alvarez-Errico, Maria Luciana Negro, Natalia Lago, Simó Schwartz Jr, Antonio Villaverde and Joan Sayós. 2012. Overexpression of the immunoreceptor CD300f has a neuroprotective role in a model of acute brain injury. Brain Pathol. 22: 318–328.

4 Elena García-Fruitós, Esther Vazquez, César Diez-Gil, José Luis Corchero, Joaquin Seras-Franzoso, Imma Ratera, Jaume Veciana, Antonio Villaverde. 2012. Bacterial inclusion bodies: making gold from waste. Trends in Biotechnology, 30: 65-70.

5 Joaquin Seras-Franzoso, César Díez-Gil, Esther Vazquez, Elena García-Fruitós, Rafael Cubarsi, Imma Ratera, Jaume Veciana, Antonio Villaverde, 2012. Synergistic bioadhesiveness and efficient mechanotransduction stimuli provided by bacterial inclusion bodies as scaffolds for tissue engineering. Nanomedicine. 7: 79-93.

6 Escarlata Rodríguez-Carmona, Olivia Cano-Garrido, Martin Dragosits, Michael Maurer, Alexander Mader, Renate Kunert, Diethard Mattanovich, Antonio Villaverde, Felícitas Vázquez.

2012.Recombinant Fab expression and secretion in Escherichia coli continuous culture at medium cell densities: influence of temperature. Process Biochemistry, 47: 446–452.

7 Gonzalez, Pau; Peluffo, Hugo; Acarin, Laia; Villaverde, Antonio; Gonzalez, Berta; Castellano, Bernardo. 2012. Interleukin-10 overexpression does not synergize with the neuroprotective action of RGD-containing vectors after postnatal brain excitotoxicity but modulates the main inflammatory cell responses. J Neurosci Res. 90: 143-159.

8 Joaquin Seras-Franzoso, Roman Affentranger, Mario Ferrer-Navarro, Xavier Daura, Antonio Villaverde, and Elena Garcia-Fruitos. 2012. Disulfide bond formation and activation of Escherichia coli ß-galactosidase under oxidizing conditions. Applied and Enviromental Microbiology, 78:2376-2385.

9 Sans, C. García-Fruitós, E, Ferraz, Rosa M, González-Montalbán, N, Rinas, Ursula, López-Santín, J, Villaverde, A and Alvaro, G. 2012. Inclusion bodies of fuculose-1-phosphate aldolase as stable and reusable biocatalysts. Biotechnology Progress 28: 421-427.

10 Esther Vazquez, José L Corchero,  Joan F. Burgueño, Joaquin Seras-Franzoso, Ana Kosoy, Ramon Bosser, Rosa Mendoza, Joan Marc Martínez-Láinez, Ursula Rinas, Ester Fernandez, Lluis Ruiz, Elena García-Fruitós and Antonio Villaverde. 2012. Functional inclusion bodies produced in bacteria as naturally occurring nanopills for advanced cell therapies. Advanced Materials, 24: 1742-1747.

FRONSTISPIECE in Advanced Materials.

NEWS in Materials Today 2012; 15: 185.

11 José L. Corchero, Rosa Mendoza, Neus Ferrer-Miralles, Anna Montràs, Lluís M. Martínez, and Antonio Villaverde. 2012. Enzymatic characterization of highly stable human alpha-galactosidase A displayed on magnetic particles. Biochemical Engineering Journal. 67: 20-27.

12 Antonio Villaverde, Elena García-Fruitós, Ursula Rinas, Joaquin Seras-Franzoso, Ana Kosoy, José Luis Corchero and Esther Vazquez. 2012. Packaging protein drugs as bacterial inclusion bodies for therapeutic applications. Microb Cell Fact, 11:76.

13 Antonio Villaverde. 2012. Bacterial inclusion bodies: an emerging platform for drug delivery and cell therapy. Nanomedicine 7: 1277-1279.

14 Ugutz Unzueta, María Virtudes Céspedes, Neus Ferrer-Miralles, Isolda Casanova,  Juan Cedano, José Luis Corchero, Joan Domingo-Espín, Antonio Villaverde, Ramón Mangues, Esther Vázquez. 2012. Intracellular CXCR4+ cell targeting with T22-empowered protein-only nanoparticles. International Journal of Nanomedicine 7: 4533 - 4544.

15 Joan Domingo-Espín, Valérie Petegnief, Núria de Vera, Paolo Saccardo, Ugutz Unzueta, Esther Vazquez, Juan Cedano, Luciana Negro, Hugo Peluffo, Anna M. Planas, Antonio Villaverde, Neus Ferrer-Miralles. 2012. RGD-based cell ligands for cell-targeted drug delivery act as potent trophic factors. Nanomedicine: Nanotechnology, Biology, and Medicine 8: 1263-1266.

16 Ugutz Unzueta, Neus Ferrer-Miralles, Juan Cedano, Xu Zikung, Mireia Pesarrodona, Paolo Saccardo, Elena García-Fruitós, Joan Domingo-Espín, Pradeep Kumar, Kailash C. Gupta, Ramón Mangues, Antonio Villaverde and Esther Vazquez. 2012. Non amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles. Biomaterials 33: 8714-8722.

17 Sang Yup Lee, Diethard Mattanovich and Antonio Villaverde. 2012. Systems metabolic engineering, industrial biotechnology and microbial cell factories. Microbial Cell Factories 2012, 11:156.

18 Hugo Peluffo, Pau Gonzalez, Laia Acarin, Anna Arís, Rudy Beyaert, Antonio Villaverde, Berta Gonzalez. 2012. Overexpression of the nuclear factor kappa B inhibitor A20 is neurotoxic after an excitotoxic injury to the immature rat brain. 2012 Neurological Research. DOI: http://dx.doi.org/10.1179/1743132812Y.0000000139.

 

2013

1 Benelmekki M, Caparros C, Xuriguera E, Lanceros-Mendez S, Rodriguez-Carmona E, Mendoza R, Corchero JL, Martinez LM.  Improving the binding capacity of Ni2+ decorated porous magnetic silica spheres for histidine-rich protein separation. Colloids Surf B Biointerfaces. 2013 Jan 1;101:370-5. doi:  10.1016/j.colsurfb.2012.07.014. Epub 2012 Jul 21

2 José Luis Corchero, Brigitte Gasser, David Resina, Wesley Smith, Ermenegilda Parrilli, Felícitas Vázquez, Ibane Abasolo, Maria Giuliani, Jussi Jäntti, Pau Ferrer, Markku Saloheimo, Diethard Mattanovich, Simó Schwartz Jr, Luisa Tutino, Antonio Villaverde. 2013. Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics. Biotechnology Advances. 31: 140–153.

3 Joaquin Seras-Franzoso, Karl Peebo, José Luis Corchero, Monica P. Tsimbouri, Ugutz Unzueta, Ursula Rinas, Matthew J. Dalby, Esther Vazquez, Elena García-Fruitós, Antonio Villaverde.  2013. A nanostructured bacterial bio-scaffold for the sustained bottom-up delivery of protein drugs. Nanomedicine 8:1587-1599.

4 Olivia Cano-Garrido, Escarlata Rodríguez-Carmona, César Díez-Gil, Esther Vázquez, Elisa Elizondo, Rafael Cubarsi, Joaquin Seras-Franzoso, José Luis Corchero, Ursula Rinas, Nora Ventosa, Imma Ratera, Jaume Veciana, Antonio Villaverde, Elena García-Fruitós. 2013. Sponge-like supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies. Acta Biomaterialia. 9: 6134–6142.

5 Joaquin Seras-Franzoso, P. Monica Tsimbouri, Karl V. Burgess, Ugutz Unzueta, Elena Garcia-Fruitos, Esther Vazquez, Antonio Villaverde and Matthew J. Dalby. 2013. Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies decorating polycaprolactone surfaces. Nanomedicine. Apr 30. [Epub ahead of print].

6 Villaverde A. 2013. Annual acknowledgement of manuscript reviewers. Microb Cell Fact. 2013, 12:11. (Editorial).

7 Witold I. Tatkiewicz, Joaquim Seras-Franzoso, Elena García-Fruitos, Esther Vazquez, Nora Ventosa, Karl Peebo, Imma Ratera, Antonio Villaverde, and Jaume Veciana. 2013. 2D microscale engineering of novel protein-based nanomaterial for cell guidance. ACS Nano. 7:4774-784.

8 Neus Ferrer-Miralles, Escarlata Rodríguez-Carmona, José Luis Corchero, Elena García-Fruitós, Esther Vázquez, Antonio Villaverde. 2013. Engineering protein self-assembling in protein-based nanomedicines for drug delivery and gene therapy. Critical Reviews on Biotechnology. Oct 9. [Epub ahead of print].

9 Joaquin Seras-Franzoso, Christoph Steuer, Mònica Roldán, Meritxell Vendrell, Carla Vidaurre-Agut, Anna Tarruella, Laura Saldaña, Nuria Vilaboa, Marc Parera, Elisa Elizondo, Imma Ratera, Nora Ventosa, Jaume Veciana, Alberto J. Campillo-Fernández, Elena García-Fruitós, Esther Vázquez, Antonio Villaverde. 2013. Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery. Journal of Controlled Release. 171:63-72.

10 Ventosa, Nora; Cabrera, Ingrid; Elizondo, Elisa; Esteban, Olga; Corchero, José; Mergarejo, Marta; Pulido, Daniel; Cordoba, Alba; Moreno-Calvo, Evelyn; Unzueta, Ugutz; Vazquez, Esther; Abasolo, Ibane; Schwartz, Simo; Villaverde, Antonio; Albericio, Fernando; Royo, Miriam; Garcia-Parajo, Maria; Veciana, JaumeMultifunctional nanovesicle-bioactive conjugates prepared by a one-step scalable method using CO2-expanded solvents. 2103. Nano Letters, 13: 3766-3774.

11 Unzueta U, Saccardo P, Ferrer-Miralles N, Cortés F, García-Fruitós E, Mangues R, Vazquez E, Villaverde A. 2013. Improved performance of protein-based recombinant gene therapy vehicles by tuning downstream procedures. Biotechnol Prog. doi: 10.1002/btpr.1798. [Epub ahead of print].

12 Vazquez, E. and Villaverde A. 2013. Microbial biofabrication for Nanomedicine: biomaterials, nanoparticles and beyond (Editorial). Nanomedicine, 8(12):1895-1898.

13 Ferrer-Miralles, N and Villaverde A. 2013. Bacterial cell factories for recombinant protein production; expanding the catalogue (Editorial). Microbial Cell Factories 12:113.

14 Ugutz Unzueta, Paolo Saccardo, Joan Domingo-Espín, Juan Cedano, Oscar Conchillo-Solé, Elena García-Fruitós, María Virtudes Céspedes, José Luis Corchero, Xavier Daura, Ramón Mangues, Neus Ferrer-Miralles, Antonio Villaverde, and Esther Vázquez. 2013. Sheltering DNA in self-organizing, protein-only nano-shells as artificial viruses for gene delivery. Nanomedicine: Nanotechnology, Biology, and Medicine Nov 21. pii: S1549-9634(13)00595-9. doi: 10.1016/j.nano.2013.11.006. [Epub ahead of print].

15 Joaquin Seras-Franzoso, Karl Peebo, Elena García-Fruitós, Esther Vázquez, Ursula Rinas, Antonio Villaverde. 2013. Improving protein delivery of Fibroblast Growth Factor-2 from bacterial Inclusion Bodies used as cell culture substrates. Acta Biomaterialia. Dec 18. pii: S1742-7061(13)00617-X. doi: 10.1016/j.actbio.2013.12.021. [Epub ahead of print].

 

2014

1 Patricia Alamo, Alberto Gallardo, Miguel Angel Pavón, Isolda Casanova, Manuel Trias, Maria Antonia Mangues, Esther Vazquez, Antonio Villaverde, Maria Virtudes Cespedes, Ramon Mangues. 2014. Subcutaneous preconditioning increases invasion and metastatic dissemination in colorectal cancer models. Disease Models & Mechanisms 7:387-396.

2 María Virtudes Céspedes , Ugutz Unzueta , Witold Tatkiewicz , Alejandro Sánchez-Chardi , Oscar Conchillo-Solé , Patricia Álamo , Zhikun Xu , Isolda Casanova , José Luis Corchero , Mireia Pesarrodona, Juan Cedano , Xavier Daura , Imma Ratera ,Jaume Veciana , Neus Ferrer-Miralles , Esther Vazquez , Antonio Villaverde , and Ramon Mangues. 2014. In vivo Architectonic Stability of Fully de novo-Designed Protein-Only Nanoparticles. ACS Nano, Just Accepted Manuscript. DOI: 10.1021/nn4055732